There could be a demonic entity in the new world. It might be awakened by Gilgamesh's arrival. This entity is used to having its way in the world and sees Gilgamesh as a new obstacle. It could possess other beings to attack Gilgamesh and use the chaos of the new world to its advantage.
The devils who oppose Gilgamesh's presence in the Highschool DxD world could be main antagonists. They might see him as a threat to their power and influence in the school.
In a magical world, a powerful sorcerer could be the antagonist. This sorcerer views Tony's attempts to blend technology and magic as an abomination and will do everything in his power to stop Tony from spreading his ideas and inventions. He might even try to steal Tony's technology for his own dark magic purposes.
Well, it could be that Gilgamesh is transported to a modern world. He might struggle with the new technology and social norms. For example, he could be baffled by smartphones and the concept of social media. Maybe he uses his ancient wisdom and strength to become a sort of protector in this new world, facing off against new threats that are a mix of modern evils and magical creatures that exist in this new world due to some strange rift between dimensions.
In a world where Naruto is a fish out of water, the local bullies or those who uphold the unfair traditions of the world could be the antagonists. They might oppose Naruto when he tries to change things for the better.
It could be a group of rogue entities that are not fully part of the new pantheon but exist on its fringes. They might oppose Percy's presence because they fear he will disrupt the balance that they are trying to manipulate for their own gain.
It could be a group of corrupt business people. They use their wealth and influence to manipulate the city's economy and politics for their own gain. Naruto, with his sense of justice, would oppose them. For instance, they might be trying to close down a community center that helps underprivileged kids, and Naruto steps in to stop them.
In a more futuristic world, a corrupt corporate leader could be the bad guy. He wants to use Naruto's abilities for his own profit, like making Naruto fight in illegal gladiator - like battles for entertainment. If Naruto refuses, he threatens to harm the people Naruto has grown to care about in this new world.
A group of female ninjas who oppose Naruto's presence in their world could be antagonists. They might believe that his arrival will disrupt their way of life and power structures. For example, a powerful all - female clan that has long held sway in the ninja world might see Naruto as a threat to their dominance.
A new student who is overly competitive and tries to undermine Ginger at every turn could be an antagonist. This student might be jealous of Ginger's popularity or her skills in a particular area like academics or a club activity.
The Yonko would definitely be major antagonists. Since Luffy is in the New World, the likes of Kaido or Big Mom would be natural choices. Their vast power and influence would pose a great threat to Luffy and his crew.