Well, in such a fanfiction, there could be a lot of interesting plot developments. Naruto, now as a girl, might face different challenges in the ninja world. For example, she could experience different treatment from her male comrades. Maybe she'd have to deal with new forms of discrimination or over - protection. Also, her relationships with other characters like Sasuke and Sakura would change significantly. Sasuke might view her in a whole new light, and Sakura could become either more competitive or more protective.
In this fanfiction, the role of Mikoto is also very interesting. She has the power to change Sasuke's gender, which implies a certain level of control or influence. It could be that she has some ulterior motives for doing this. Maybe she wants to see how Naruto and Sasuke's relationship will develop in this new situation, or perhaps it's part of a larger plan within the fanfiction's universe.
One possible plotline could be Naruto having to deal with the new social dynamics as a girl. For example, she might face different expectations from her friends and sensei. Another could be how she adapts to her new physical form during training, like how her changed body affects her chakra control. And also, there could be a romantic sub - plot where characters who were just friends before now see her in a different light.
Well, in many fanfictions, Naruto might use his ninja skills. He could use his speed to reach Artemis quickly if she's in danger, like if she's being attacked by some powerful enemy. His shadow clone jutsu could also be useful to distract the foes while he rescues her.
The impact could be huge. It could change the power dynamics in the story. As a girl, Naruto might be underestimated by some opponents at first, giving her an advantage in battles. It could also lead to changes in the way the plot unfolds. For example, some missions might be approached differently due to her new identity.
Themes in 'naruto x sasuke turned girl fanfiction' can also include the alteration of power dynamics. Usually, Sasuke is a very powerful male character. But as a girl, the way she wields her power and how others perceive it can change. It might lead to situations where Naruto has to be more protective, or where female Sasuke has to prove herself more in a male - dominated ninja world. And of course, there's always the underlying theme of how their bond, which was so central in the original series, evolves in this new, gender - flipped context.
One interesting plot could be Naruto, as a girl, having to deal with the different expectations of the ninja world towards females. For example, she might face challenges in being taken seriously in combat training at first. Another plot could involve Naruto's friends' reactions. Some might be really confused, while others could quickly accept and support 'her'.
Sasuke would definitely be one of the main characters to interact with Naruto. Their relationship has always been complex, and with Naruto as a girl, it could become even more so. Sakura would also interact a lot. She might be both jealous and intrigued by Naruto's new form. Kakashi too, as Naruto's mentor, would have to adjust to this change and guide 'her' in a different way perhaps.
Sasuke would be really interesting. His relationship with Naruto was always complex, and if Naruto is a girl, it could add a new level of tension or even a different kind of friendship/rivalry. Sakura too. She might have a whole new perspective on Naruto as a girl, and they could form a stronger bond as female comrades. And Kakashi, his reaction to Naruto's transformation could be quite humorous and also a source of guidance for Naruto in this new situation.
Another possibility is that it's part of a scientific experiment that backfires. Xander now has to deal with the social implications of being a girl, like different expectations in school or in relationships. He might also find that some things are easier as a girl, such as getting away with certain behaviors because of different gender stereotypes.