Well, perhaps the human could be transported into the My Little Pony world and gradually take on the characteristics of Twilight. Maybe they start by learning magic like Twilight does, and as they do, they become more like her in personality too, like becoming more studious and dedicated to knowledge.
It could be a dark magic user who doesn't want the human - turned - Twilight to succeed. Maybe this antagonist sees the new Twilight as a threat to their own power or plans in the pony world.
Another theme is friendship. Just like in the original My Little Pony series, Twilight forms a bond with the human character. They support each other through various challenges, whether it's Twilight helping the human understand a magical problem or the human helping Twilight with a non - magical issue in their world.
A cool concept for 'human becomes twilight fanfiction' is a human who gets transported into the Twilight universe. This person could try to change the story's events. Maybe they befriend Bella and try to prevent some of the dangerous situations she gets into. Or they could end up falling in love with a minor character and create a whole new sub - plot. It could also be that they are turned into a hybrid of a vampire and a werewolf, which is a unique situation not really explored in the original series.
To make it unique, you could change the setting. Instead of the typical Forks, set it in a big city like New York or Paris. The human's transformation would then be influenced by the different environment. For example, hiding their new vampire or werewolf nature would be more challenging in a crowded city. Another way is to give the human a special ability that is not in the original Twilight. Maybe they can control the weather or have telepathic powers over all supernatural beings.
Her friends like Pinkie Pie, Rainbow Dash, Applejack, Rarity and Fluttershy are likely to be main characters. Pinkie Pie might bring her usual cheer and parties to celebrate Twilight's new form. Rainbow Dash could be both impressed and a bit competitive, trying to keep up with Twilight's new abilities in her own way. Applejack would offer her down - to - earth advice and support. Rarity might be excited to create new outfits for Twilight as an alicorn. And Fluttershy would be there with her gentle nature, providing a calming presence.
There's also 'Twilight and the Human Scholar'. In this fanfiction, Twilight meets a human scholar who is interested in Equestrian magic. Their relationship grows as they exchange knowledge, and it delves into how their different worlds' cultures and magics interact.
You can start by looking on fanfiction websites such as Archive of Our Own (AO3). It has a large collection of fanfics, and you can search specifically for 'mlp twilight human fanfic' using the search bar. Just make sure to read the tags and summaries to find the ones that interest you.
One popular 'mlp twilight human fanfic' might be those that explore Twilight's life as a human in a modern-day high school setting. For example, she could be the new girl who is really smart but a bit of an outcast because of her intense focus on her studies, similar to her studious nature in the pony world.
One interesting plot could be Twilight's struggle to adapt to her new alicorn form. She might face challenges in controlling her enhanced powers at first. For example, she could accidentally cause magical outbursts while trying to perform simple spells. Another plot could involve how her relationships with her friends change. Some friends might be in awe of her new status, while others could be a bit intimidated initially.