In certain comic continuities, Gwen Stacy is alive. However, her status has changed throughout different comic arcs and reboots. Sometimes she's been brought back through various means in the fictional comic world.
Gwen Stacy is not trans in the comics. The character has been presented in a conventional gender role throughout the comic series. There have been no storylines suggesting otherwise.
Gwen Stacy was an important figure in the Spider-Man comic universe. She was smart, kind, and had a complex relationship with Peter Parker. Her death had a huge impact on the story.
Peter Parker is one of the main characters. He has a deep connection with Gwen. Her father is also important. He's often concerned about Gwen's safety in the dangerous world of the comic. And there are the villains like the Green Goblin who play a major role in the story.