It could be some dark wizards who have somehow gotten hold of the nuke and plan to use it for their evil purposes. They might be in league with some Muggle villains who know about nuclear technology.
Well, in this 'Harry disarms a nuke fanfic', Harry could first need to find the nuke. Maybe he gets a tip from a magical creature or uses his invisibility cloak to approach it undetected. Once he locates it, he has to figure out how to use his magic on non - magical technology. His quick thinking and bravery would be crucial in this out - of - the - ordinary situation.
There could also be a new group of supernatural beings, perhaps a cult that worships dark magic. They might target Hellsing because of their power and the fact that Harry, with his wizarding abilities, is now part of the organization. This cult could be trying to summon some ancient and extremely dangerous entity, and Hellsing, with Harry's help, has to stop them.
There could be a secret society within the wizarding world that has long opposed the Knight Templars. They see Harry's new - found connection to them as a revival of an old threat. They try to undermine Harry and his attempts to bring the Templar values into the wizarding world by spreading false rumors and creating obstacles for him at every turn.
Some pure - blood wizarding families who value strict traditions and order might oppose Harry. They might view his very existence as an affront to their way of life. They could try to use their influence to drive him out of the wizarding community or plot against him in secret.
The main antagonists could be the top - ranking officials in the Ministry who are desperate to hold on to their power. They would likely use all means at their disposal, including dark magic and underhanded tactics, to stop Harry and his allies.
There could also be a group of dark mages who want to use the chaos of this inter - dimensional relationship for their own gain. They might try to manipulate Harry and Serafall's families and friends against the marriage, and then when things are at their worst, try to steal their combined magical power which they believe will be extremely potent when Harry and Serafall are united through marriage.
There could also be new antagonists introduced, perhaps some old - fashioned wizards who oppose the idea of a girl being in the position of power or being the 'chosen one'. They might try to undermine Harry at every turn, not just because of her role in the prophecy but also because of her gender.
Obviously, Thanos himself could be an antagonist at times. Since he's Harry's father, there could be a power struggle between them, with Thanos wanting Harry to follow his ways strictly. Another antagonist could be those who oppose Harry just because of his relation to Thanos. They might not trust him and try to eliminate him, thinking he'll be as evil as his father.
One possible antagonist could be a jealous professor who feels threatened by Harry's popularity among students. This professor might try to undermine Harry's teaching methods and reputation. Another could be a student who has fallen under the influence of a dark magic book in the college library and starts causing trouble. Or it could be an old enemy from Harry's past who has disguised themselves and managed to get a job at the college to seek revenge on Harry.
It could be a new group of dark wizards or witches who have emerged with a different kind of dark magic. Maybe they are not directly related to Voldemort but have their own evil motives. They could be after a powerful magical artifact at Hogwarts, and their attacks prompt Harry to invent the Protegah spell.