Perhaps Bellatrix Lestrange had a secret accomplice who was also interested in the Potter family. After Bellatrix's death, this accomplice decides to hunt down the missing sister to finish what Bellatrix started - destroying the Potter family once and for all.
Another option is a power - hungry Ministry official who discovers the existence of Harry's missing sister. He believes that by controlling her, he can gain more influence and power within the wizarding world. So he uses all his resources to find her before Harry does, and will stop at nothing to achieve his goal.
Well, it could be that the sister was kidnapped by dark wizards when she was a baby. Years later, Harry discovers some mysterious clues that lead him to believe she might still be alive. He then embarks on a dangerous journey to find her, facing many magical obstacles along the way.
It could be a new character created by the fanfiction author. Maybe a powerful wizard who was once shunned by the wizarding community and now wants revenge by stealing magic.
A dark wizard who has merged the dark arts with Wonderland's magic could be the main villain. He might have created some sort of dark Wonderland where all the creatures are under his control, and Harry has to break this control to restore balance.
Perhaps the main villain is actually a corrupted version of a well - known character. For example, if Hermione was somehow influenced by the dark magic associated with the runestone path and turned against Harry. It would be a very unexpected and heart - wrenching twist, as she has always been such a loyal friend. But the power of the runestone path could be so strong that it changes her fundamental nature.
Maybe a new character, a Muggle - born wizard who was shunned by both the Muggle and wizarding worlds. This character, out of bitterness, decides to combine Muggle technology and magic to create the droid army and rule over both worlds. He would see Harry Potter and his friends as the main obstacles to his grand plan.
It could be someone new, a dark wizard who has learned about Naruto's reincarnation and wants to use his power for evil. This new villain might try to manipulate Naruto into using his chakra in a wrong way. Or perhaps it could be a combination of Voldemort and some of Naruto's old enemies from his previous life who somehow find a way to merge their powers in the Harry Potter world.
Perhaps an old Hufflepuff alumni who has turned to the dark side. This person was once a great student but became corrupted over time. They return to Hogwarts with the intention of using the Hufflepuff house as a base for their dark plans. They manipulate the Hufflepuff students, taking advantage of their kindness and hard - working nature, and turn it into a means to an end for their evil deeds. Harry gets caught up in this as he tries to figure out who this mysterious villain is and stop their plans.
A possible main villain could be Voldemort who somehow gains knowledge of Naruto's world and uses it to his advantage. He could learn to use chakra and combine it with his dark magic. He might create some sort of hybrid minions that are part - Death Eater and part - ninja. This would make him even more powerful and a greater threat to both the wizarding world and Naruto's world.
A possible main villain could be a dark wizard who has learned some of the criminal tactics from the Detective Conan world. He could be using both magic and devious non - magical schemes to cause chaos. For instance, he might use spells to steal valuable items and then use the underworld connections in Conan's world to sell them.
Maybe the main villain could be a corrupted Hogwarts professor. This professor could be secretly teaching dark magic to students and when Harry's child discovers this, they become the target. The professor wants to stop the child from revealing their dark secrets and will go to great lengths to do so, making them a formidable villain in the fanfiction.