Maybe a witch who has a vendetta against vampires in general. She could use her magic to cause all sorts of trouble for Edward, like casting spells that prevent him from using his vampire abilities or even creating illusions to drive him mad. Since she's a witch, she has different powers compared to a normal vampire hunter, so she'd be a very formidable antagonist. She might also be able to manipulate other humans or vampires to do her bidding in her quest to get rid of Edward.
An ancient vampire who has been awakened from a long slumber could be the main antagonist. This vampire has old - fashioned and more brutal ways, and sees Edward's more modern approach to vampirism as a threat. He wants to assert his dominance and bring back the 'old ways' of being a vampire, which would involve a lot of bloodshed and chaos, directly opposing Edward's attempts to co - exist with humans.
A powerful and corrupt politician could be the main antagonist. He might be using his influence to cover up illegal activities, and Edward, as an honest cop, stands in his way. The politician could try to frame Edward or have him removed from the case.
It could be another vampire clan. They might see Cartman and Kyle's relationship as a threat to their own power or way of life. For example, if Cartman and Kyle are trying to promote co - existence between vampires and humans, the other clan might oppose it.
It could be a character who is jealous of the relationship between Roy and Edward. Maybe someone who wanted to adopt Edward themselves or has a grudge against Roy for taking Edward in.
It could be one of the traditional Volturi members who oppose Edward's presence. They might see him as an outsider who could disrupt their long - established order. For example, Caius might be against Edward joining as he's often portrayed as more rigid and less accepting of new vampires in the Volturi.
A new vampire who is jealous of Edward's relationship with Bella could be the antagonist. With Edward having amnesia, this new vampire could try to take advantage of the situation and win Bella over. They could also try to turn Edward into a more 'evil' version of himself while he is vulnerable.
Perhaps a half - alien, half - vampire hybrid. This character was shunned by both species and blames Ben for making him feel even more like an outcast. He uses his unique powers to create chaos and tries to turn Ben into a vampire - alien like himself.
Perhaps a powerful vampire lord who sees Sookie as a threat to his power. Since she was once human and has connections to both worlds, he might fear that she could rally other vampires and humans against him. He could try to eliminate her by any means necessary, leading to a lot of action - packed scenes in the fanfiction.
It could be an old and powerful vampire who sees Hermione as a threat to his rule. Since Hermione is a very intelligent witch - now vampire - she might be able to figure out a way to undermine his power.
It could be an old and powerful vampire lord who sees Natsu as a threat to his rule. Since Natsu is a new and different kind of vampire, the lord might want to eliminate him.
Maybe the main antagonist is a scientist who has been experimenting with vampire-like powers and accidentally creates a powerful vampire - like monster. This monster then starts attacking the world, and Shadow, as a new vampire - hedgehog, has to stop it. The scientist might also be trying to cover up his mistake and will stop at nothing to keep Shadow from interfering.