The main antagonist could be a jealous character who is envious of Bertha's unique features, like her 'big boob'. This character might try to use Bertha's hiccup problem against her, perhaps by making her hiccup in embarrassing situations to lower her self - esteem.
It could be an evil merchant who wants to capture Bertha because of her unique appearance. He sees her 'big boob' as something that can be exploited for profit. He uses the hiccup as a way to track her down. Every time she hiccups, it gives off a small signal that he can detect. Lemon then has to figure out a way to stop the merchant from getting to Bertha while also trying to find a cure for the hiccup.
One possible plot could be that Bertha, who has a large figure, has an unexpected hiccuping problem that she tries to hide. Meanwhile, Lemon, a friend, discovers it and tries to help her in a comical way. Hiccup could be some sort of magical or strange occurrence that adds to the humor of the story.
It could be a dragon hunter who sees Hiccup in his dragon form and wants to capture him for study or to use his unique abilities for evil purposes.
A rogue dragon rider who has different ideas about how to treat dragons. He might see them as tools for power rather than friends like Hiccup does. This rider could be causing trouble for Hiccup and his tribe by stealing dragons or using them to attack other tribes.
Maybe a rival Viking tribe that has a different view on dragons. They see dragons as a threat rather than friends like Hiccup and Valka do. So they try to disrupt the peace that Hiccup and Valka are trying to build between the Vikings and the dragons. They could be constantly causing trouble, stealing dragon eggs or attacking dragons, which would force Hiccup and Valka to take action against them.
A power - hungry Viking who sees Hiccup's transformation as a threat to his own authority could be the main antagonist. He might try to capture Hiccup in his Night Fury form and use him for his own evil purposes, like trying to create an army of transformed creatures.
It could be a lemon - loving pirate who has a grudge against Luffy for some reason. Maybe Luffy accidentally destroyed his precious lemon grove on an island. So this pirate, with his crew of lemon - themed fighters, constantly tries to sabotage Luffy's adventures and steal his treasures.
Maybe a powerful warlord who rules over the region where the winter lemons grow. He sees Naruto as a threat to his control over the valuable lemon resources and decides to eliminate him. The warlord might have an army of minions and some dark magic of his own that he uses against Naruto and his friends.
Lex Luthor could be a great antagonist. He might see Rogue Lemon as a new variable in his plans to defeat Superman. He could try to manipulate Rogue Lemon into turning against Superman or use her powers for his own evil purposes.
It could be a dark wizard who has somehow managed to control the Xenomorphs and is using them to cause chaos. This wizard would see Harry as a threat to his plans and would constantly send the Xenomorphs after him, creating those 'lemon' - like difficult and dangerous situations for Harry.
Maybe a giant lemon - themed monster. This monster is created by some dark force that wants to disrupt the balance between the two worlds in the crossover. It has the ability to spit out acidic lemon juice that can corrode anything. Ichigo has to figure out its weakness and defeat it to restore peace. The monster could be controlled by an unseen force that is the real antagonist behind the scenes.