Another possibility is that a Death Eater could get hold of the Tensaiga and misuse it. This would turn that Death Eater into a more formidable opponent. Harry would then have to not only fight against the dark magic of the Death Eater but also figure out how to take back the Tensaiga and stop it from causing more harm.
It could still be Voldemort. Even with the introduction of the Tensaiga, Voldemort would remain a major threat. He might become even more interested in the Tensaiga once he learns about it, seeing it as a powerful tool to achieve his dark goals.
The discovery of the Tensaiga in the fanfiction could also lead to some cultural clashes. Since the Tensaiga comes from a different fictional universe, Harry might struggle to understand its origin and purpose. He could seek help from Hermione, who would start researching about it. This could involve trips to the library, consulting with magical creatures, and in the end, they might find a way to incorporate the Tensaiga's power into their fight against the Death Eaters.
Maybe it could be a dark wizard who also discovers chakra but uses it for evil purposes. He sees Harry as a threat because Harry wants to use chakra for good and to protect both the wizarding world and the world where chakra exists.
Perhaps a long - lost relative of the Potter family who feels they were unjustly excluded from inheriting the manor. This relative could be jealous of Harry's status and the wealth associated with the manor. They might use dark magic to try and claim what they think is rightfully theirs, leading to a battle of magic and loyalty within the Potter family and among Harry's friends.
A new dark wizard or witch could be created specifically for this fanfiction. This character could be interested in using the Huntsman's skills for their own evil purposes. If the Huntsman refuses, they become enemies. This antagonist could have powers related to dark forest magic, something that the Huntsman is familiar with but in a more malevolent form.
Voldemort could still be the main antagonist. Even if Demetri has some sort of connection or change in the fanfiction, Voldemort's overarching evil plans would still be a major threat to both Harry and Demetri, especially if Demetri tries to deviate from the Death Eaters' path.
The minotaur itself could be the main antagonist. It's a powerful and dangerous creature, and if it's been set loose in Hogwarts or is guarding something that Harry needs, it would be a major obstacle for him to overcome.
The main antagonist could be a dark wizard who has made the graveyard his lair. He uses the dark energy from the tombstones and the restless spirits there to power his dark magic. Harry stumbles upon this wizard's activities and has to stop him.
A new dark wizard or witch could be the main antagonist. This character, jealous of Harry's fame and power, creates a situation where Harry is framed for a heinous crime. They then ensure his arrest so that they can take over the role of the 'chosen one' in the eyes of the wizarding public and start spreading their own brand of dark magic.
It could be Malfoy. He might make fun of Harry for wearing a dress and try to use it to humiliate him in front of the whole school, which would then lead to a conflict between them that goes beyond the usual rivalry.
Perhaps a new dark wizard or witch who has infiltrated the Ministry of Magic and made sure Harry was sent to Azkaban. This antagonist could be working towards a bigger plan to take over the wizarding world and sees Harry as a major threat even when he's locked up.