Perhaps a powerful and unknown shinobi from a far - off land. This shinobi has heard of Team 7's reputation and sees them as a threat to his plans of world domination. He uses his unique and mysterious jutsu to challenge Team 7 at every turn, and they have to figure out his motives and weaknesses to stop him.
It could be Orochimaru. He has always been interested in Sasuke and might target ANBU Team 7 to get to him. Orochimaru's experiments and his desire for power make him a formidable and menacing villain. He could also be using some new jutsu or have created more powerful minions to fight against Team 7.
A new character could be created as the main villain. Maybe a rogue ANBU agent who was once a friend of Naruto and Sasuke but has been corrupted by power. This character would know all the ins and outs of the ANBU, making it very difficult for Naruto and Sasuke to stop him as he tries to take over the ninja villages using the secrets he learned while in the ANBU.
Perhaps a powerful ninja from another village who has a vendetta against Konoha and the ANBU. They see the ANBU's connection with Naruto as a threat and use their knowledge of the lemon fanfiction element in some way. For example, they might create clones that are disguised as lemon - scented ANBU members to infiltrate Konoha and cause chaos.
Maybe in the fanfic, Team 7 in ANBU is tasked with protecting a powerful and important shinobi from another village who has come to Konoha for peace negotiations. Naruto tries to befriend the visiting shinobi, Sasuke is constantly on guard against any possible threats, and Sakura is ready to heal any injuries in case of an attack. There could be internal conflicts within Team 7 as they deal with different approaches to protection.
You might consider adding Tenten to the team. She has a vast knowledge of weapons which could be handy in various missions. And Kiba, with Akamaru by his side, could offer enhanced senses and tracking abilities for the Anbu team.
Perhaps a powerful shinobi from another village who has a special grudge against the Leaf's ANBU. He targets Kakashi and Naruto specifically because of their reputation within the ANBU. He has unique abilities that pose a great challenge to them, like the ability to nullify ninjutsu for short periods.
The main antagonist could be an old - school ninja who believes in the traditional and often harsh ways of the ANBU. They see Naruto's actions as weakening the village and set out to oppose him at every turn, trying to bring back the old ANBU regime.
Well, one major plot change could be in the power dynamics. Since Naruto in Anbu is likely more skilled and experienced. He might take a more dominant role in Team 7's missions. For example, he could quickly assess and handle dangerous situations that the original Team 7 might struggle with. This could also change the relationships within the team. Sasuke might be more competitive towards Naruto, seeing him as an even greater rival. And Sakura might have more admiration or fear towards Naruto because of his Anbu status.
Perhaps an evil organization that wants to capture Ino for her unique mind - related abilities. They believe they can use her powers for their own malicious purposes. Naruto and the rest of the team then have to protect Ino and take on this organization. The leader of this group could be the main villain, with his own set of powerful and dark jutsu.
Obviously, Naruto is one of the main characters. He is the central figure in this fanfic as it's about him in the ANBU. There may also be other characters like Kakashi. Since Kakashi has experience in the ANBU, he could play an important role in guiding Naruto or being involved in some of the missions with him.