In such a fanfiction, the plot could involve Ash being betrayed by his long - time friends. Maybe they envy his success in the Pokemon world. For example, Gary could spread false rumors about Ash in the new region, causing the local trainers to distrust Ash. This would make Ash's journey in the new region much more difficult as he has to not only deal with new Pokemon and challenges but also regain his reputation.
One possible plot could be that the Pokemon League in the new region, for some reason, frames Ash for cheating. They could claim he used illegal Pokemon or items during battles. This betrayal by the very organization that should uphold fairness would force Ash to prove his innocence. He would have to go on a quest to find evidence to clear his name while facing the scorn of the other trainers in the new region.
It could be his rivals. For instance, Tracey or Paul might see Ash's potential in the new region as a threat and decide to betray him. They could try to sabotage his battles by giving false tips to his opponents or stealing his battle strategies.
Perhaps in this new region, Ash discovers a powerful new Pokemon that others want to control. His friends, fearing the power of this Pokemon, betray him to protect the region. They might think that Ash is being too reckless with this new discovery and that it could lead to destruction. It could also be that there's a prophecy in the new region that Ash is somehow involved in, and his friends misinterpret it and believe he's the cause of potential harm.
Ash would probably be really hurt at first. He's always been so trusting of his friends. He might go off on his own for a while to clear his head, like he did when he had a falling - out with some of his friends in the past.
One possible plot could be that within the fanfiction world, there is a new character introduced who seems to be a great ally at first. This character gains the trust of Ash and Brock over time. However, this new character has a hidden agenda. They might be from a rival region and their goal is to undermine Ash and Brock's reputation in the Pokemon world. So they start spreading false rumors about them and turn other trainers against them, thus betraying their new - found friendship.
Well, perhaps Clemont betrays Ash because he becomes envious of Ash's success. Maybe he gets influenced by some dark force in the Pokemon world that convinces him to steal Ash's Pokemon or to lead him into a dangerous trap.
It could be that Lillie betrays Ash because she believes it's the only way to achieve a greater good in the Pokémon world. She might think that Ash's methods are too dangerous or not in line with what she believes is right for the future of Pokémon and humans alike. This would create a complex conflict between their ideologies.
Well, in many such fanfictions, it could involve Ash being betrayed by Clair perhaps in a Pokémon battle strategy. Maybe Clair teams up with an unexpected rival against Ash, or she might break a promise regarding Pokémon training or a joint adventure.
In such a fanfiction, perhaps Ash could be betrayed by his friends in Alola. Maybe they misunderstand his actions or are influenced by some false information. For example, they might think Ash is secretly working for an enemy, which leads to the betrayal.
One interesting idea for the fanfiction is that May betrays Ash to protect her family. An evil group threatens her family and the only way to save them is to turn against Ash. This would create a complex situation where May is torn between her loyalty to Ash and her love for her family. Ash, on the other hand, would be completely blindsided by her sudden change in behavior.
It could be that Mew has some sort of special power or knowledge that certain groups in the Pokémon world are after. They see Ash as an obstacle to getting what they want from Mew. So, they scheme to make everyone think Ash is untrustworthy. His friends, not knowing the truth, end up betraying him. Ash then has to fight against all odds to protect Mew and regain the trust of his friends.