Developing characters in a Totally Spies OC harem fanfic requires a lot of thought. Firstly, for the OC, consider their appearance. If they have a unique style, like always wearing a vintage hat, it can be a talking point. Their values are also important. If they value honesty above all else, it will come into play when dealing with the spies who sometimes have to lie for their missions. For the spies, show their growth. Maybe Sam becomes more open - minded because of the OC, Clover learns to be less materialistic, and Alex becomes more patient. These character developments can be intertwined with the harem - like relationships in the fanfic.
For the OC, give them a distinct personality. For example, if they're shy, it'll contrast with the confident spies. Also, define their goals. If they want to be a great scientist, it can interact with the spies' spy - work. For the spies, show how their existing personalities react to the OC. Sam might be more nurturing towards a younger OC, while Clover could be competitive at first but then grow fond. Alex could be the one who tries to understand the OC's unique situation deeply.