In addition to the usual suspects, there could be some new characters introduced. For example, an old Pokémon trainer who has seen similar things before and can offer some advice on how to deal with baby Ash. Or a mysterious Pokémon that has the power to either help or hinder the process of getting Ash back to normal.
Another possible main character could be a wise old mentor figure. This person might have knowledge about these strange transformations. They could guide Nico's friends or family in their quest to turn Nico back. Maybe this mentor has been around for a long time and has seen similar magic used in the past, so they know where to start looking for answers.
One idea could be that a powerful magic spell backfires on Ash. His friends then have to take care of baby Ash while trying to find a way to reverse the spell. They might face challenges like getting baby Ash to eat Pokémon food or stop him from toddling towards dangerous Pokémon.
Another possibility is that the main antagonist is an evil scientist who was responsible for Ash's transformation in the first place. This scientist could be trying to create some sort of chaos in the Pokémon world by turning a well - known trainer like Ash into a girl. And now, Ash as a girl has to stop this scientist from causing more harm to the Pokémon world.
The Akatsuki might be the main antagonists. They might think that a baby Sasuke is easier to capture and they could use him for their own purposes, like extracting his Sharingan or something else related to his power. They would be a great threat as they are powerful and cunning.
It could be an old rival of the Malfoy family. Maybe a wizard whose family was wronged by the Malfoys in the past. This wizard has been biding his time, waiting for the perfect opportunity to strike. Turning Draco into a baby is his way of getting revenge on the family, making them look weak and powerless.
Perhaps an old enemy of Harry's family resurfaces. This villain might have a long - standing grudge against the Potters and sees turning Harry into a baby as a form of ultimate revenge. They could be using a spell that has been passed down through generations in their family, a spell that was created specifically to harm the Potters.
Definitely Aunt May. She would be super worried about baby Peter and would do everything she could to take care of him. She might even call in some old friends or neighbors to help out.
Sakura could be a main character. She might be the one who takes on a motherly role towards baby Naruto. She'd be very protective and try to teach him basic things. Kakashi could also be important. He'd use his wisdom to figure out how to deal with this situation and also keep an eye on Naruto's safety.
Obviously, Nightwing himself, even as a baby, would be a main character. His friends in the Bat - family like Batman, Robin, and Oracle would also be main characters. They would be the ones dealing with the situation of baby Nightwing.
It could be Team Rocket as always. Since Ash is now a Pikachu, they might see it as an easy target to capture. They could have new gadgets specifically designed to catch Pikachu - Ash, and constantly try to get their hands on him throughout the fanfiction.