If it's in a work - related setting, his boss or colleagues could be significant characters. His boss might be understanding and give him time off to recover, or his colleagues could be worried about how his absence will affect the work, which could create some interesting plot points.
Well, aside from John the Baptist, there's Jesus, of course. Jesus is crucial because John's whole mission was to pave the way for Jesus' ministry. And then there's Herodias. She was Herod Antipas' wife, and she had a vendetta against John the Baptist which contributed to his downfall. Herod Antipas himself was also important. He was the ruler who made the fateful decision regarding John's life.
In this fanfiction, perhaps John catches a mysterious illness that has strange symptoms. It could be a magical malady in a fantasy setting, like a curse that makes him age rapidly.
One of Tobirama's students, like Kakashi's father, Sakumo Hatake, could be a main character. He would be eager to prove himself by finding a way to heal his teacher. He could face many challenges and grow as a character throughout the process of trying to save Tobirama.
Even the kids who visit the pizzeria could be main characters. They might be the first to notice that Foxy is acting strange. One of the kids could be really attached to Foxy and be very worried about him. This kid could try to get the staff's attention to help Foxy or even try to comfort Foxy on their own.
Annie John herself is the most important character. She is the protagonist whose experiences and growth form the core of the story. Her mother is also very important as their relationship is central to many of the novel's themes.
Alphys could be another common character. As the royal scientist, she might be called upon to figure out what's wrong with Sans. She could bring her scientific knowledge to the table, trying to diagnose and find a cure. Her own insecurities might play a role here too, as she might be worried about failing to help Sans.
A friend or a family member of Jaime could be significant. They could be there to offer emotional support, or they might have their own opinions about the new hand. Their relationship with Jaime could be tested and strengthened throughout the story.
His friends like Batman and Nightwing could be main characters. Batman might use his detective skills to figure out what's wrong with Robin, and Nightwing could be there to offer emotional support.
Since Rigby is sick, his family members could also be main characters in the fanfiction. They might come to visit him and bring their own unique family drama to the story. And his friends from the park, like Muscle Man and High Five Ghost, could also be involved, trying to cheer him up while he's ill.
John is obviously one of the most important characters. He's right there in the title. His friend, whose name I can't recall at the moment but is also central to the story. And then there are all those strange creatures and entities that they interact with. They play important roles in creating the crazy and dangerous situations that John and his friend have to face.