Maybe a jealous rival from Ash's past who has been biding their time. They could befriend Leaf and slowly turn her against Ash by filling her head with lies about how Ash has always been one - step ahead of them unfairly. This would make the rival the main antagonist driving the betrayal plot.
It could be a new and powerful trainer who has a vendetta against Ash. This trainer might see Ash as a threat to their own rise to the top in the Pokemon world. They could manipulate Leaf into betraying Ash to further their own goals.
An evil Pokemon organization, like Team Rocket but more menacing, could be the main antagonist. They might target Ash because of his unique bond with his Pokemon. They could use Leaf's insecurities or desires to make her betray Ash, for example, by offering her a powerful Pokemon in exchange for her cooperation.
It could be a jealous rival trainer. This trainer might have always been envious of Ash's popularity and the loyalty of his harem. So, they plot and scheme to turn the harem against Ash by spreading lies and creating discord among them.
It could be a powerful and jealous Galar trainer. This trainer might see Ash as a threat to their own reputation and success in the region. They would then use their influence and charm to turn Ash's friends against him.
It could be a new and powerful trainer who manipulates Anabel into betraying Ash. This trainer might be jealous of Ash's success and see Anabel as a means to bring Ash down.
A powerful and envious Pokémon trainer could be the main antagonist. This trainer might be jealous of Ash's bond with Greninja and decides to break it. He could use dark magic or some sort of mind - controlling device to make Greninja turn against Ash.
It could be a new character who is jealous of Ash and Skyla's friendship. This character might spread false rumors about Ash to Skyla, causing her to feel betrayed. So, this new jealous character would be the main antagonist.
The Pokemon Professor in the region could also be the antagonist. Suppose the professor discovers a rare and powerful Pokemon that Ash is also interested in. The professor, driven by greed or a desire for power, might betray Ash by making it seem like Ash is trying to steal the Pokemon, causing all of Ash's friends and the community to turn against him.
Maybe there is an evil Pokemon trainer who has influenced Ashley to betray Ash. In this case, that trainer would be the main antagonist. He or she might have promised Ashley something in return for her betrayal, like rare Pokemon or power.
It could be a powerful and evil Pokemon trainer who wants to capture Ash in his new Pokemon form. This trainer might be after Ash because he believes that a human - turned - Pokemon has some special power or value.
Maybe a powerful and evil pokemon trainer who has a vendetta against Ash. Now that Ash is in a vulnerable pokemon form, this trainer could try to capture and control him. The trainer might have some dark motives related to using Ash's unique situation to gain power in the pokemon world.
A powerful and greedy Team Rocket member could be the main antagonist. They might be after Diancie because of its rare and valuable powers. They could try to capture Diancie and use it for their own evil purposes, like selling its powers or using it to take over the Pokemon world.