Perhaps a jealous classmate. This classmate was always in the spotlight before Jaden turned into a girl. But now, with Jaden getting all the attention because of this strange transformation, the classmate feels left out and decides to be the antagonist. They might try to sabotage Jaden's new friendships or chances to participate in school activities.
It's possible that the main antagonist could be an old enemy of Jaden who now sees this transformation as a chance for revenge. This enemy might try to expose Jaden's secret to the whole school in a cruel way, thinking that it will make Jaden an outcast. But in the end, Jaden's true friends stand by him/her and they all face the enemy together.
It could be a new villain who is specifically targeting Gohan because of his new form. This villain might see Gohan's transformation as a sign of weakness or an opportunity to mess with the Z - fighters. Maybe they want to use Gohan's confusion and the chaos it causes among his friends and family to their advantage.
It could be someone who wants to take advantage of Minato's new female form. Maybe a villain who has a vendetta against Minato in his original form and sees this as an opportunity to further humiliate or harm him.
Another possibility is that a group of Koopa minions could be the main antagonists. They might be under the orders of Bowser, but they have their own little schemes going on. They could target Mario - turned - girl because they think she'll be an easier target in her new form, but they are in for a big surprise when she shows her strength.
The Joker could still be the main antagonist. He might find the idea of Batman being a girl extremely hilarious and try to use it to his advantage. Maybe he would try to play mind games with her, thinking that she would be more vulnerable in her new form.
The main antagonist could be a jealous classmate. This classmate has always been envious of Marco's popularity or abilities. When Marco turns into a girl, this antagonist sees it as an opportunity to make his life miserable. They might spread rumors or try to embarrass Marco in his new form.
Bowser could be the main antagonist. He might see Luigi's transformation as an opportunity to mess with Mario and the Mushroom Kingdom. He could try to use Luigi's new form to his advantage, perhaps by trying to make Luigi a spy or by using his new identity to cause chaos among the kingdom's residents.
Ganon could still be the main antagonist. His motives remain the same - to take over Hyrule. But now he might underestimate Link because she's a girl, which could be his downfall.
It could be a jealous rival. Maybe someone who has always been envious of Robin's abilities or popularity. Now that Robin is in a more vulnerable state as a girl, this rival sees an opportunity to undermine him.
It could be a witch who cast the spell that turned Dean into a girl. This witch might have some ulterior motives, like using Dean's new form to disrupt the balance in the hunting world. Maybe she wants to create chaos among hunters by making them question Dean's abilities now that he's a girl.
Another possibility is Snape. Snape might be suspicious of Ron's transformation and think it's some sort of dark magic at play. He could constantly be on Ron's case, trying to figure out what happened and making her life difficult in potions class and around the school in general. He might also suspect that Ron is up to something that could disrupt the order at Hogwarts.