It could be another dark mage who sees the potential in baby Natsu and wants to use him for evil purposes. This mage might view Zeref as an obstacle in getting to Natsu.
Maybe an ancient evil entity that has been awakened by the presence of both baby Natsu and Zeref. It wants to destroy them both to prevent some future event that they are destined to cause.
There is a possibility that a group of anti - magic zealots could be the antagonists. They see Zeref as a symbol of all that is wrong with magic and they fear what baby Natsu could become under Zeref's influence. So they try to separate them and destroy any chance of their relationship developing further.
It could be a dark wizard who wants to use the baby's power for his own evil deeds. Maybe the baby has some special magic that the wizard thinks can help him take over the magical world.
Natsu and fem Zeref are the main characters. Natsu, who is always ready for a fight and loves his friends deeply. In this fanfiction, his interactions with fem Zeref would be crucial. Fem Zeref, she could be portrayed in different ways. Maybe she is more empathetic than the original Zeref or has different magical abilities. Their combined presence drives the story forward, as the fanfiction would likely explore how they interact with each other and the world around them.
Maybe some unexpected allies could emerge. For instance, other dark mages who oppose Zeref's actions for their own reasons. They might see Natsu as a valuable asset or simply dislike Zeref's overbearing ways. Another potential ally could be a powerful spirit that Natsu has befriended in the past. Spirits often have unique powers that could be useful in the fight against Zeref.
It could be a dark wizard who wants to use Natsu's connection to the Dragonoid for his own evil purposes. This wizard might have set the trap to either steal Natsu's power or to use him as a sacrifice to gain more power related to the Dragonoid.
It could be an old and powerful vampire lord who sees Natsu as a threat to his rule. Since Natsu is a new and different kind of vampire, the lord might want to eliminate him.
It could be a long - lost relative of an old enemy. For example, if there was a powerful dark wizard that Natsu defeated in the past, this relative might come seeking revenge and be powerful enough to kill Natsu.
Maybe an ancient dragon that has been awakened from its slumber and views Natsu as an intruder in the dragon world. Since Natsu is a new - made dragon, this old dragon might try to drive him out or even harm him.
Maybe there's a dark wizard who has a vendetta against Natsu's family. When he finds out that Natsu is secretly a girl, he sees it as an opportunity to further his evil plans. He could try to kidnap her or use her as a bargaining chip against her family or friends. He might think that because she's a girl, she'll be easier to manipulate or capture compared to if she was thought to be male.
Another vampire family that is jealous of the Mikaelson's power and influence. They see the baby as a threat to their future plans. They might try to kidnap the baby and use it as a bargaining chip to gain more power in the vampire world. They would be constantly plotting against the Mikaelsons to get their hands on the baby.
Danzo could be a great antagonist even when Naruto is a baby. He might see Naruto as a threat to his plans for Konoha's future and try to get rid of him secretly while making it look like an accident.