It could be a powerful dark wizard who has found a way to control the forces in the Land of the Dead. Maybe he was once a student at Hogwarts who delved too deep into the Dark Arts and now wants to use the power of the dead to rule the wizarding world.
Perhaps an ancient and evil spirit that has been trapped in the Land of the Dead for centuries. This spirit is awakened by some dark magic and decides to break free and wreak havoc on the living world. It sees Harry Potter and his friends as a threat and tries to stop them from interfering with its plans. It has the ability to possess the dead and make them do its bidding, which makes it a very dangerous antagonist.
The main antagonist could be a Death Eater who survived the battle against Voldemort. This Death Eater, in his desperation for power, has found a way to enter the Land of the Dead and has made a deal with the dark entities there. He returns with new and terrifying powers, and his goal is to finish what Voldemort started. He targets Harry Potter because he knows that Harry is the biggest obstacle in his path to ruling the wizarding world.
Well, it could be about Harry and his friends having to journey into the Land of the Dead to retrieve a powerful magical artifact. Maybe they need it to defeat a new dark wizard who is using the power of the dead to gain strength.
Perhaps a long - lost relative of the Potter family who feels they were unjustly excluded from inheriting the manor. This relative could be jealous of Harry's status and the wealth associated with the manor. They might use dark magic to try and claim what they think is rightfully theirs, leading to a battle of magic and loyalty within the Potter family and among Harry's friends.
A new dark wizard or witch could be created specifically for this fanfiction. This character could be interested in using the Huntsman's skills for their own evil purposes. If the Huntsman refuses, they become enemies. This antagonist could have powers related to dark forest magic, something that the Huntsman is familiar with but in a more malevolent form.
Voldemort could still be the main antagonist. Even if Demetri has some sort of connection or change in the fanfiction, Voldemort's overarching evil plans would still be a major threat to both Harry and Demetri, especially if Demetri tries to deviate from the Death Eaters' path.
The minotaur itself could be the main antagonist. It's a powerful and dangerous creature, and if it's been set loose in Hogwarts or is guarding something that Harry needs, it would be a major obstacle for him to overcome.
The main antagonist could be a dark wizard who has made the graveyard his lair. He uses the dark energy from the tombstones and the restless spirits there to power his dark magic. Harry stumbles upon this wizard's activities and has to stop him.
A new dark wizard or witch could be the main antagonist. This character, jealous of Harry's fame and power, creates a situation where Harry is framed for a heinous crime. They then ensure his arrest so that they can take over the role of the 'chosen one' in the eyes of the wizarding public and start spreading their own brand of dark magic.
It could be Malfoy. He might make fun of Harry for wearing a dress and try to use it to humiliate him in front of the whole school, which would then lead to a conflict between them that goes beyond the usual rivalry.
Perhaps a new dark wizard or witch who has infiltrated the Ministry of Magic and made sure Harry was sent to Azkaban. This antagonist could be working towards a bigger plan to take over the wizarding world and sees Harry as a major threat even when he's locked up.
Maybe a dark wizard who has an interest in Harry's newfound inheritance. This antagonist could want to steal his powers or use him for their own evil purposes. They might see Harry as a key to unlocking some ancient and powerful magic that they've been seeking.