An ancient and malevolent Pokemon that has awakened from a long slumber could be the antagonist. It sees Ash, now in his Pokemon form, as a threat or as a source of power it can absorb. This Pokemon would use its dark powers to try and defeat Ash and take over the Pokemon world.
A new, powerful and evil Pokemon trainer could be the antagonist. This trainer might be jealous of Ash's new form and powers as a Pokemon and wants to capture him to use his powers for his own gain. He could set up various traps and battles to try and subdue Ash.
A powerful and greedy Pokémon collector could be the main antagonist. He wants to capture Ash, now in his Mew form, to add to his collection. He has all sorts of high - tech devices and trained Pokémon to try and catch Ash.
Team Rocket could still be the main antagonists. They might see Ash as Pikachu and think it's a new, rare Pikachu. So they try to capture him more desperately than ever. They could have some new gadgets designed specifically to catch this 'strange' Pikachu which is actually Ash.
It could be a powerful and evil Pokemon trainer who wants to capture Ash in his new Pokemon form. This trainer might be after Ash because he believes that a human - turned - Pokemon has some special power or value.
Maybe a powerful and evil pokemon trainer who has a vendetta against Ash. Now that Ash is in a vulnerable pokemon form, this trainer could try to capture and control him. The trainer might have some dark motives related to using Ash's unique situation to gain power in the pokemon world.
One possible plot could be that Ash is transformed into a rare and powerful Pokemon like Mewtwo. He then has to navigate the Pokemon world from a different perspective. He discovers new abilities and strengths he didn't know about before. And he uses his knowledge of being a trainer to communicate with other Pokemon and form alliances to face the challenges that come his way.
A powerful and greedy Team Rocket member could be the main antagonist. They might be after Diancie because of its rare and valuable powers. They could try to capture Diancie and use it for their own evil purposes, like selling its powers or using it to take over the Pokemon world.
A wealthy and power - hungry trainer who wants to stop Ash from completing his Nuzlocke journey. This trainer believes that the Nuzlocke rules are too restrictive and wants to prove that his way of training Pokemon is superior.
It could be a rival trainer who is jealous of Ash's success and also wants to use Princess Sara for her influence. He spreads false rumors about Ash to turn the people of the princess's kingdom against him. He then tries to take over the role of the hero in the situation, but his real intentions are to gain power and wealth by any means necessary.
It could be Team Rocket. They are always after rare and powerful pokemon. In this fanfiction, they might have a new and devious plan to steal the legendary pokemon that Ash is trying to catch. Their comical yet persistent attempts to thwart Ash would add an interesting dynamic to the story.
Maybe a new villain organization that believes in a different way of training Pokemon, a cruel and unethical way. They see Ash as a threat to their ideology because he is a symbol of the traditional, kind - hearted Pokemon training. So they constantly try to stop him from achieving his goal of becoming a Pokemon master. For example, they might try to steal his Pokemon or sabotage his battles.