One possible plot twist could be that Zeref has an ulterior motive for joining. Maybe he wants to use the guild's resources to further his own dark plans.
Perhaps a plot twist would be that Zeref joining Fairy Tail causes a rift in the magical world. Other guilds or dark forces might see this as a threat and start to target Fairy Tail. This would force the guild to either defend Zeref or give him up, creating a lot of drama and action in the fanfiction.
One possible plot twist could be that Zeref actually doesn't want to fight Natsu seriously and has some hidden agenda. Another could be that Natsu discovers a new power during the fight that is related to his past or his true identity.
Well, it could be chaos. Zeref's dark powers would likely disrupt the harmony of Fairy Tail at first. His presence might cause a lot of suspicion and fear among the guild members.
A possible plot twist could be that Zeref's dark power resurfaces during the wedding. Maybe an old enemy takes advantage of the situation and tries to turn Zeref against Mavis. But Mavis' love and the power of Fairy Tail's friendship save the day.
Most of the Fairy Tail members would not take it well. Happy, Natsu's closest friend, would be extremely confused and sad. He'd follow Natsu around, trying to get him to come back to the guild. Makarov, the guild master, would see it as a huge threat to the guild's safety. He might send some of the stronger members to keep an eye on Natsu and Zeref. And the younger members like Wendy would look up to Natsu so much that they'd be very disappointed and scared for him, thinking that he's making a big mistake by joining Zeref.
Another plot could involve Irene using her unique powers to help Fairy Tail in a big crisis. Maybe there's a powerful enemy threatening the guild and her abilities are crucial in the fight. For example, she could use her magic to turn the tide in a battle against a dark guild.
One idea could be that Zeref and Mavis are somehow trapped in a parallel dimension. In this dimension, they have to work together to find a way out. Along the way, they start to understand each other's pasts and the pain they've both endured. This could lead to a newfound respect between them.
Another plot twist could be that the past they remember isn't actually real. It was all an illusion created by a powerful mage. When they find out, they have to figure out who the real enemy is and why they were put in this false past. This would completely shift the focus of the fanfiction from their relationship in the 'past' to finding out the truth behind the illusion.
Another plot twist could be related to his journey of self - discovery. As a guy reborn as Lucy, he has to come to terms with his new identity. He might struggle with the physical and emotional aspects of being a female in the Fairy Tail universe. This internal conflict could drive the story forward as he tries to find his place within the guild and the world at large, all while dealing with the challenges that come with being Lucy Heartfilia.
Natsu could develop a more empathetic side. Knowing Zeref as his brother, he might start to understand the pain and loneliness that Zeref has endured. Zeref, on the other hand, could become more open and less isolated. He might find a new purpose in protecting Natsu and the Fairy Tail guild. For example, Zeref could start to use his powers for good instead of causing destruction.
Perhaps a plotline where an outside force tries to manipulate Natsu and Zeref against each other while they are in Fairy Tail. This could lead to a lot of internal conflict within the guild as members take sides. But in the end, through the power of friendship and the values of Fairy Tail, they see through the ruse and become stronger together, uniting the guild even more firmly.