Applejack and the Heavy might be the first to interact. Applejack is a hard - working and practical character. The Heavy is a no - nonsense fighter. Applejack might see the Heavy and be curious about his large size and strange weapon. The Heavy, on the other hand, might be interested in Applejack's farming skills and the fresh apples she might be carrying. Their down - to - earth personalities could lead to an initial interaction.
Spyro and Twilight Sparkle would be likely to interact. Twilight is very intelligent and well - versed in magic, and Spyro has his own dragon powers. They could learn from each other. For example, Twilight could study Spyro's dragon magic, and Spyro could learn about the complex magic system in Equestria from Twilight.
Mercy from Overwatch and the Medic from TF2 could be the first to interact. Given their roles as healers, they would likely be interested in each other's medical techniques. They might start by comparing notes on how to heal their comrades more effectively, and this could lead to a deeper understanding and cooperation between the two groups.
Probably Naruto and Robin. Robin is the leader - type in Young Justice, always thinking and strategizing. When Naruto shows up, Robin would be the one to approach him first, trying to figure out who he is and what his intentions are. Naruto, being friendly and curious, would be open to the interaction.
The Scout from TF2 is often popular. His high - energy and fast - paced nature can create interesting interactions with the ponies in MLP. He might be constantly running around and getting into all sorts of misadventures in the MLP world.
Probably Naruto and Tracer. Naruto is always eager to meet new people and make friends, and Tracer's curious and friendly nature would make her approach Naruto. They could start by comparing their abilities. Naruto would show off his Rasengan while Tracer might do a quick display of her blinking ability.
Probably Ash and Iron Man. Iron Man is always curious about new things and Ash with his Pokémon would be quite an interesting sight for him. He'd likely approach Ash first to ask questions about what he is and what those strange creatures are.
It could be Percy and Black Widow. Natasha is a master of reading people, and she'd notice Percy right away. She might approach him in a more stealthy way compared to the others, trying to assess if he's a threat or an ally. And Percy, being used to danger, would be on his guard at first, but then start to trust her as she shows her good intentions.
Probably Naruto and Twilight Sparkle. Twilight is the intelligent and curious one in the My Little Pony world, always eager to learn new things. Naruto, with his unique ninja abilities, would be something completely new for her. She would likely approach Naruto first to find out more about him.
Probably Spongebob and Twilight Sparkle. Spongebob is always curious and eager to explore new things, and Twilight is the most studious and open - minded of the ponies. So when Spongebob arrives in Equestria, Twilight would be the one most likely to approach him first, wanting to learn about this strange new creature. And Spongebob would be happy to talk to her because she seems friendly and intelligent.
I think Pikachu and Fluttershy would be likely to interact first. Pikachu is curious and friendly, and Fluttershy is gentle and kind - hearted. Their personalities would make them approach each other easily.