It's also possible that there are human characters who interact with the bee world in the novel. For instance, a beekeeper or a scientist studying the bees could be among the main characters, and their perspective could add an interesting dimension to the story as they observe and interact with the queen bee and her hive.
As I haven't read it, I can only guess. Obviously, the queen bee is likely to be a main character. There might also be worker bees that play important roles, perhaps as her loyal subjects or as characters with their own sub - plots. Maybe there are other insects or even animals that interact with the queen bee and are considered main characters as well.
The main theme could be about power and leadership, as a queen bee is often associated with ruling a hive.
There's no way to tell exactly without reading it. But common main characters in a 'bee graphic novel' could be the bees. The queen bee might be a central figure, ruling over the hive. Worker bees could be the heroes, doing all the hard work to keep the hive running. And if it has a more creative twist, there could be a bee that is different from the others, like a bee with the ability to communicate with humans, and that bee could be a main character.
There could be her best friend who supports her journey to become the 'queen bee'. Maybe someone named Lily who is always there to give Chloe advice on her outfits and how to handle social situations.
Since it's a honey bee graphic novel, the main characters are likely to be different types of bees. The queen bee would be a central figure as she is responsible for laying eggs and leading the hive. Worker bees could also be main characters as they perform all the tasks in the hive like foraging, building the hive, and taking care of the brood. There might also be a brave drone that has some special mission or adventure within the story.
The main characters are Bee and PuppyCat. Bee is a young woman who is kind of lost in life, and PuppyCat is, well, a strange creature that looks like a puppy and a cat combined. They have a great chemistry together.
The main characters are surely Phineas and Ferb. They are the ones who always embark on these amazing adventures. And of course, their sister Candace who is often trying to bust them for their activities. Also, Perry the Platypus who has his own secret agent life and might be involved in the 'bee day' story in some way, maybe protecting the boys from some threat related to the bees or the bee - related situation they get into.
The main characters are surely the little bees. They are the ones around which all the stories revolve.
I'm not sure which novel you're referring to because you didn't provide any relevant information. If you can provide the name of the novel or provide more context, I will be more than happy to provide you with a more accurate answer.
Another possible theme is the cycle of life. Since the queen bee is involved in reproduction in the beehive, the story could be centered around the idea of new life, growth, and the continuation of the colony. It might show how the queen bee's role is crucial in this cycle, from laying eggs to the development of new bees.