There could be an ancient evil force that has been dormant for a long time. The formation of the harem between Naruto and Moria Queen awakens this force as it senses the newfound power and unity. This antagonist would be extremely powerful and would pose a great threat to not only the harem members but also the entire world they are in, forcing them to work together to defeat it.
One possible plotline could be that Naruto finds himself in a new world where he meets Moria Queen and gradually forms a harem around her. He might use his ninja skills to protect the group from various threats in this new world. Another plotline could involve Naruto teaching his ninja values to the members of the harem, leading to personal growth for all of them.
One possible antagonist could be a powerful wizard or sorcerer who is jealous of Naruto's growing influence and the harem he has. This antagonist might try to break up the group and steal their powers.
One likely main antagonist could be a powerful demon lord who is trying to break through the barriers between the spirit world and the human world. This demon lord would be a major threat to Naruto and his harem, and they would have to use all their combined skills to stop it.
The main antagonists could be a group of rogue ninjas. They target Naruto because of his reputation as a banished and potentially vulnerable target. And they also see Fem Asuma and her group as an obstacle. They might try to kidnap the female characters to lure Naruto into a trap, or steal valuable resources from them to undermine their growing community.
A jealous classmate could be a main antagonist. This person might be envious of Naruto's popularity and his ability to make friends easily. They could try to spread rumors or sabotage Naruto's projects.
The Akatsuki could still be a major antagonist. Even if Naruto is emperor, they might oppose his rule as it goes against their own goals of chaos and obtaining the tailed beasts.
One possible antagonist could be a jealous noble from Naruto's own kingdom who feels that Naruto doesn't deserve the prince title. This person might try to undermine Naruto at every turn, spreading false rumors about him or trying to sabotage his diplomatic efforts.
Another possible antagonist could be a fellow inmate who has a personal vendetta against Naruto. Maybe this inmate was once on the wrong side of Naruto's actions outside the prison and now sees this as an opportunity for revenge. He could try to undermine Naruto at every turn, from spreading false rumors about him to physically attacking him when the guards aren't looking.
Another option could be Orochimaru. He has his own motives and is always looking for power. He could be the head of a powerful gang in the criminal underworld of the Naruto world. He would constantly be trying to capture Naruto or his friends to use them for his experiments or to gain more power over the ninja world.
The Akatsuki could still be the main antagonists. They might be interested in Naruto's tails for their own dark purposes, like using their power to create a super - weapon or gain control over the ninja world. Another option could be a secret group of rogue ninjas who have a vendetta against the Uzumaki clan and see Naruto's tails as a symbol of their power that they want to destroy.