It could be the Akatsuki. Even though Itachi was once part of them, now that he has the power of a Jinchuriki, they might see him as a threat. They could want to capture him to either extract the tailed beast or to stop him from using its power against them.
Another possible main antagonist could be rogue ninja who are after the power of the tailed beast inside Itachi. They might form a group and launch attacks on Itachi and those he protects. These rogues could be jealous of his newfound power and want it for themselves to gain dominance in the ninja world.
Well, the plot could involve Itachi having to deal with the power of the tailed beast within him. Maybe he has to constantly struggle to control it while still maintaining his calm and collected exterior. He could use the power in unexpected ways to protect his village or those he cares about, like Naruto does with the Nine - Tails.
It could be a group of rogue ninjas who want to use the power of the Monster King for their own evil purposes. They might try to capture the Monster King and force it to do their bidding, which would put them in direct conflict with Naruto.
Another possible antagonist could be a character from Naruto's own world who has also been brought to this new world and has been corrupted by the power of the Witch King. This character might envy Naruto's new - found power as the Witch King and wants to take it for himself. So, he teams up with the dark forces in this new world to oppose Naruto, creating a lot of challenges for Naruto to overcome.
The White Walkers could be major antagonists. Naruto would have to use his skills to combat their undead hordes. Their magic is very different from the chakra - based abilities Naruto has, so it would be a great challenge.
The Akatsuki could still be a major antagonist. Even if Naruto is emperor, they might oppose his rule as it goes against their own goals of chaos and obtaining the tailed beasts.
One possible antagonist could be a jealous noble from Naruto's own kingdom who feels that Naruto doesn't deserve the prince title. This person might try to undermine Naruto at every turn, spreading false rumors about him or trying to sabotage his diplomatic efforts.
Another possible antagonist could be a fellow inmate who has a personal vendetta against Naruto. Maybe this inmate was once on the wrong side of Naruto's actions outside the prison and now sees this as an opportunity for revenge. He could try to undermine Naruto at every turn, from spreading false rumors about him to physically attacking him when the guards aren't looking.
Another option could be Orochimaru. He has his own motives and is always looking for power. He could be the head of a powerful gang in the criminal underworld of the Naruto world. He would constantly be trying to capture Naruto or his friends to use them for his experiments or to gain more power over the ninja world.
The Akatsuki could still be the main antagonists. They might be interested in Naruto's tails for their own dark purposes, like using their power to create a super - weapon or gain control over the ninja world. Another option could be a secret group of rogue ninjas who have a vendetta against the Uzumaki clan and see Naruto's tails as a symbol of their power that they want to destroy.
One potential antagonist could be a new and mysterious organization that is similar to Akatsuki but with different motives. They might be interested in using Daisuke to open a portal to a different dimension or something like that. They would be constantly trying to kidnap Daisuke and force him to cooperate. Also, Orochimaru or his remaining followers could be antagonists. Even though Orochimaru has changed somewhat in the series, there could still be some of his followers who are more radical and want to use Daisuke for their dark experiments, believing that he has some special chakra or ability that could help them achieve their twisted goals.