The Joker would probably be both delighted and a bit confused. He's used to Batman being his nemesis, but an evil Batman would change the dynamic. He might initially see it as a new and exciting game, but then realize that an evil Batman could be a real threat to his own chaotic existence. The Joker might try to manipulate the situation to his advantage, but it could easily backfire on him.
The other members of the Bat - family, like Nightwing and Batgirl, would be in shock at first. They would initially not believe that Batman could turn evil. But once they realize it's true, they would try to stop him. They might try to find a cure if it's a case of mind control or try to reason with him if it's a psychological shift. However, they would also be conflicted because he was their mentor and friend, and they would be reluctant to use lethal force against him.
Batman would be heartbroken. He has trained Robin and sees him as a partner and almost like a son. He would first try to talk to Robin, to find out what made him turn evil. He would hope that he could reason with Robin and bring him back to the good side.
The other Pokemon in the Pokemon Center where Pikachu is usually treated would be very frightened. Nurse Joy would be at a loss as to what to do, as she has never seen Pikachu act this way. Professor Oak would be extremely concerned and would start researching immediately to find out what could have caused this sudden change in Pikachu. Team Rocket, on the other hand, might initially see this as an opportunity to capture Pikachu more easily, but they would soon realize that an evil Pikachu is far more dangerous than they expected and might end up getting in over their heads.
The villains would have a mixed reaction. Some of the more power - hungry ones might see this as an opportunity to join forces with Saitama and cause even more chaos. However, there would also be villains who are afraid of Saitama's power, even if he's on their side now. They would be worried that he could turn on them at any moment. Overall, Saitama turning evil would completely disrupt the existing power dynamics in the One Punch Man world.
Zazu would be flustered. He's used to reporting to the grown - up Simba and giving advice. With Simba as a baby, he would be at a loss for a while. He would try to maintain order in the pride but would also be constantly worried about Simba's safety. The hyenas, if they got wind of this, would see it as a chance to cause chaos. They might try to attack the pride while they think Simba is vulnerable. But the pride, united by their love for Simba (even in his baby form), would be ready to defend their king and their territory.
Tails would probably be very curious and eager to figure out how to change Sonic back. He might study Sonic's new form scientifically. Knuckles, on the other hand, would be a bit more confused but still stand by Sonic's side. Amy might be initially jealous because she's used to being the main female around Sonic, but then she could become a great friend and help Sonic adjust. Dr. Eggman would likely see this as an opportunity to take advantage of Sonic's confusion in her new form.
Germany might be really confused at first. He's used to Prussia as his older brother figure, so seeing Prussia as a girl would be a big shock. He might be a bit protective in a different way than before. Italy would probably be very excited and might even start flirting a bit. He's always been a bit of a ladies' man, so he'd be intrigued by this new female Prussia.
Jiraiya would have a very interesting reaction. Since he's a bit of a pervert in the series, he would have to really adjust his behavior. He might also see this as an opportunity to study the differences in chakra and fighting styles between male and female ninjas while also trying to guide Naruto in this new form.
Some characters might be very shocked at first. For example, Sandstorm, if she was in a relationship with Firestar before, would be confused and have to re - evaluate her feelings. But others, like Graystripe, might be more accepting and just see it as another change that they have to adapt to. Younger cats in the clan would probably be more curious and excited about this new Firestar.
Rukia would probably be one of the first to be both shocked and amused. She might tease Ichigo a bit at first but then quickly get serious about helping him. Chad would be very accepting and offer his protection as usual. Uryu might be a bit more skeptical at first, thinking it's some kind of new Hollow trick. Orihime would be very empathetic and try to make Ichigo feel comfortable in his new form, maybe even offer to make some new clothes for him to fit his new body.
The other characters' reactions would be quite diverse. For the more competitive male players, they might see this as an advantage or a disadvantage depending on their relationship with Kirito. If they were rivals, they might think it's a chance to outdo him now that he's a girl. But if they were friends, they would offer support. Female characters like Silica might be excited to have another girl around and might start sharing girl - only things with Kirito. The guild members of Kirito's previous guilds would likely be confused but still accept him, after all, they know it's still Kirito inside.