A group of anti - superhero fanatics could be the main antagonists. They constantly try to expose Buttercup's identity and undermine her efforts to protect the city. They spread false rumors about her and make her life very difficult, causing her to question her role as a superhero.
Perhaps an old acquaintance from her past who has turned bad. This person knows all of Buttercup's weaknesses and uses that knowledge to manipulate her and create chaos in her life. They might be jealous of Buttercup's powers and popularity and want to take it all away from her.
It could be about Buttercup facing relationship troubles. Maybe she has a conflict between her superhero duties and her love life. For example, she falls in love with someone who doesn't understand her double life.
It could be a rogue ninja from another village who has a vendetta against Naruto for his past actions in his first life. This ninja might see Naruto's second chance as an opportunity to finally get revenge.
It could be Orochimaru. In this fanfic, he could be a player of the same 'life - game' as Naruto, but with a much more sinister goal. He might be trying to manipulate the game rules to his advantage, like finding a way to make all the ninja in Konoha his puppets. Naruto would then have to figure out how to stop Orochimaru's evil plans within the game - like mechanics of his world.
She could meet some other super - powered beings who are also on the run from something. Maybe they are former villains who are trying to reform.
It could be a new dark wizard who has emerged in Harry's new life. This wizard might be jealous of Harry's fame and want to take him down.
Perhaps a group of anti - technology extremists could be the main antagonists in an Ultron fanfic. They see Ultron as the symbol of all that is wrong with technology and are determined to take him down, no matter the cost. They might use their own advanced, but more rudimentary, technology to fight against Ultron. This would create an interesting contrast between Ultron's high - tech abilities and their more basic, yet effective, methods.
It could be Mr. Moseby. He might seem like he's trying to keep Cody from getting better because he doesn't want any disruptions on the ship. But in the end, it could turn out that he was just being strict to maintain order.
A powerful vampire could be the main antagonist. Vampires and werewolves are often enemies in such fictional settings. This vampire could be after Akakuro's pack's territory or have a personal vendetta against Akakuro himself.
It could be Orochimaru. He's always been interested in unique powers and abilities. The idea of X - 23's mutant abilities and Naruto's jinchuriki powers would be very appealing to him. He might try to capture them both to use in his experiments to gain ultimate power.
A possible main antagonist could be Eris, the goddess of strife. She might be jealous of Aphrodite's beauty and power and see Percy as a tool to disrupt Aphrodite's plans. Eris could send her minions to create chaos and conflict between Percy and Aphrodite, trying to make them turn against each other.