A powerful Pokémon that Ash once wronged in the past could be the main antagonist. Maybe Ash accidentally hurt this Pokémon during a battle or a quest for a rare item. Now, the Pokémon has grown stronger and is seeking revenge, forcing Ash to run from his past actions towards this Pokémon.
It could be an older version of a previous rival. For instance, Gary could have become bitter over the years and now blames Ash for something in their shared past. So he is constantly chasing Ash, making Ash run from his past connection with Gary.
It could be that Ash has some past mistakes or traumas in his Pokémon journey that he is trying to escape from. Maybe he made a wrong decision during a battle that cost him a Pokémon's trust, and now he's running from the guilt. Or perhaps there are some events related to his old rivals that he wants to avoid.
Maybe a powerful and evil pokemon trainer who has a vendetta against Ash. Now that Ash is in a vulnerable pokemon form, this trainer could try to capture and control him. The trainer might have some dark motives related to using Ash's unique situation to gain power in the pokemon world.
A powerful and greedy Team Rocket member could be the main antagonist. They might be after Diancie because of its rare and valuable powers. They could try to capture Diancie and use it for their own evil purposes, like selling its powers or using it to take over the Pokemon world.
A wealthy and power - hungry trainer who wants to stop Ash from completing his Nuzlocke journey. This trainer believes that the Nuzlocke rules are too restrictive and wants to prove that his way of training Pokemon is superior.
Maybe a new villain organization that believes in a different way of training Pokemon, a cruel and unethical way. They see Ash as a threat to their ideology because he is a symbol of the traditional, kind - hearted Pokemon training. So they constantly try to stop him from achieving his goal of becoming a Pokemon master. For example, they might try to steal his Pokemon or sabotage his battles.
It could be a new character, a powerful trainer who is jealous of Ash's natural talent and secretly tries to undermine him at every turn.
It could be a future version of Team Rocket. They might have become more powerful and devious in the future and travel back in time to stop Ash from achieving something important in the past that would prevent their future rise to power. Another option could be a time - traveling Pokemon hunter who wants to capture rare and powerful Pokemon from different time periods, and Ash stands in his way.
It could be a jealous trainer. This trainer might see Ash in his shiny pokemon form and want that power for himself. So he tries to capture Ash by any means necessary, setting up all kinds of traps and challenges for Ash and his friends.
There could be a new, evil organization in the fanfiction. Their goal is to use shiny pokemon to power some sort of dark machine or experiment. They target Ash's shiny pokemon because it has a particularly strong energy. This organization would send out their minions to attack Ash and try to capture the shiny pokemon, creating a lot of action - packed scenes in the story.
It could be a powerful and jealous trainer who wants to take Ash's place. This trainer might use dark magic or some underhanded tactics to make Ash's Pokemon abandon him so that he can swoop in and claim them for his own.