Mercy from Overwatch and Cortana from Halo might have an interesting interaction. Mercy is a healer and Cortana is an AI. They could discuss the ethics of artificial intelligence and how Mercy's healing technology could potentially be applied in the Halo universe, or vice versa how Cortana's knowledge could enhance Overwatch's medical technology.
Genji from Overwatch and Arbiter from Halo could also be a great pair to interact. Genji, with his cybernetic enhancements, and the Arbiter, with his unique leadership role among the Sangheili, could find common ground in their experiences of being different from the norm in their respective worlds and use their skills to overcome challenges together.
Definitely Ruby Rose and Master Chief. Ruby is the main character in RWBY, full of energy and determination. Master Chief is a well - known and iconic figure in Halo. Their different personalities and combat styles would create an interesting dynamic. Ruby's quick - thinking and agility could pair well with Master Chief's stoic and strategic approach in battles.
Mercy from Overwatch and the Medic from TF2 could be the first to interact. Given their roles as healers, they would likely be interested in each other's medical techniques. They might start by comparing notes on how to heal their comrades more effectively, and this could lead to a deeper understanding and cooperation between the two groups.
Probably Naruto and Tracer. Naruto is always eager to meet new people and make friends, and Tracer's curious and friendly nature would make her approach Naruto. They could start by comparing their abilities. Naruto would show off his Rasengan while Tracer might do a quick display of her blinking ability.
Probably Bloom from the Winx Club and Master Chief from Halo. Bloom is the main character in the Winx Club, very powerful and often takes the lead in dangerous situations. Master Chief is the iconic figure in Halo. Their paths might cross first because they both have a strong sense of duty and leadership. They would be likely to recognize each other's capabilities quickly and start to work together, like when facing an initial threat that appears in both of their worlds.
Yang would be a likely character. She's bold and not one to back down from a challenge. She might see Godzilla as a huge opponent to test her strength against. Maybe she'd start off trying to punch it, only to realize that brute force alone won't work and then have to figure out a more strategic way to interact with it.
Sunset Shimmer and Sonic might be the first to interact. Sunset has a bit of an edgy side and Sonic is always ready for action. If Sonic showed up in their world, Sunset would probably be the one to confront him, thinking he's a threat. But as Sonic explains his situation, they could start to form an alliance. Also, Sunset has experience with different worlds and magic, which could be useful in helping Sonic navigate the Equestria Girls' world.
I think Quinn and Black Widow would be an interesting pair. Quinn is used to being in a position of power and popularity in the Glee club, and Black Widow is a strong female character in the Avengers. They could have some really cool exchanges about strength and femininity.
I think Sakura from Naruto and Stella from Winx could interact a lot. Sakura is a strong - willed and intelligent kunoichi, and Stella is a confident and powerful fairy. They could have some really interesting conversations about their respective worlds, like comparing the medical ninjutsu in Naruto's world to the healing magic in the Winx world. And they might also get into some friendly rivalries while training together.
Bloom and Iron Man could be likely to interact. Bloom is a powerful fairy with strong leadership qualities, and Iron Man is a genius inventor and leader in the MCU. They could both exchange ideas about leadership and power, and maybe Bloom could teach Tony a thing or two about magic. Another pair could be Stella and Thor. Stella loves fashion and luxury, and Thor is from a royal background in Asgard, so they could bond over their love for grandeur.
Mercy from Overwatch and the Medic from TF2 would be likely allies. They both have a strong focus on healing. Mercy's ability to fly and revive teammates could be well - complemented by the Medic's Ubercharge ability. Another pair could be Tracer and Scout. They are both fast - moving characters. They could use their speed to scout out areas, steal intel or make quick strikes against the enemy.