A powerful dark sorceress could be the main antagonist. She might be jealous of the Queen's title and powers of love and beauty. So, she could try to steal the Queen's powers or spread chaos in the kingdom to make the Queen look bad. For example, she could curse the crops to fail or make the people fall ill, and then blame it on the Queen.
A rival queen from a neighboring kingdom could be the main antagonist. She believes that she should be the true Queen of Love and Beauty. So, she starts a series of political and magical maneuvers. She could send spies into the Queen's court, try to undermine her diplomatic relations, and even attempt to use dark magic to taint the Queen's beauty. This would lead to a great conflict between the two kingdoms and the two queens.
Obviously Arya would be the main character. But there could also be her loyal handmaidens who help her with her daily tasks and give her advice on how to be a good queen.
A power - hungry noble could be the main antagonist. This person might be jealous of the Queen's position and wants to overthrow her, and sees Harry as an obstacle in his way.
The fanfic could also revolve around Arya's love life as the Queen of Love and Beauty. She could have a secret admirer who is too shy to approach her. Meanwhile, Arya is so focused on her duties as queen that she doesn't notice at first. But as time passes, she starts to develop feelings for someone unexpected, and this new relationship causes a stir in the court.
The Volturi Queen herself could be the main antagonist. If she views Bella as a threat to her power or the Volturi's way of life, she would be a very powerful foe. She could use her influence over the Volturi guards and her own powers to try and stop Bella.
It could be an envious witch. She was once in love with the prince (now the Beast) but he rejected her. So she cursed him and now she wants to make sure he never finds true love with the Dark Beauty.
Perhaps a group of anti - technology extremists could be the main antagonists in an Ultron fanfic. They see Ultron as the symbol of all that is wrong with technology and are determined to take him down, no matter the cost. They might use their own advanced, but more rudimentary, technology to fight against Ultron. This would create an interesting contrast between Ultron's high - tech abilities and their more basic, yet effective, methods.
It could be a powerful dark wizard or sorcerer who wants to control both Naruto's world and the Spider Queen's domain. This antagonist might be after the unique powers that Naruto and the Spider Queen possess, like Naruto's chakra and the Spider Queen's web - based magic. He could try to manipulate them against each other to achieve his goals.
It could be a dark wizard who wants to disrupt the new relationship between the wizarding world and the queen. This wizard might see Harry's position as a threat to his own plans of taking over the Muggle world starting from the monarchy.
It could be a powerful demon who wants to control the Succubus Queen and use her powers for evil. Since Naruto is with the Queen, he becomes a target too.
It could be a powerful witch. This witch is jealous of the Powerpuff Girls' beauty and power. So she casts a spell that mutes their beauty, making them look ordinary. This not only affects their appearance but also weakens their powers. The witch wants to take over Townsville while the girls are in this weakened state.