One possible reason could be a divergence in their goals. Maybe in the fanfiction, Harry has a different approach to fighting Voldemort and Ron doesn't agree with it, leading to a rift. For example, Harry might want to take a more aggressive and risky approach while Ron prefers a more cautious one. This difference in ideology can drive them apart.
Another reason could be the influence of other characters. Suppose a new character enters the story and befriends Harry, but this character has a vendetta against Ron. They might manipulate Harry into believing that Ron is not trustworthy, causing Harry to distance himself from Ron.
One possible reason could be a misunderstanding. Maybe a miscommunication led to a rift between them. For example, if Harry overheard Ron saying something that was taken out of context, it could start the breakdown of their friendship.
It could be due to a misunderstanding. Suppose Ron overhears a conversation between Harry and Hermione that he misinterprets completely. He might think they are plotting against him or don't really consider him a true friend. So in a fit of anger and hurt, he decides to betray them.
It could be a misunderstanding. For example, Ron might think Harry did something really bad to Hermione or their other friends. Without proper communication, his anger could lead him to punch Harry. Also, if there was some sort of magical influence or dark magic at play that affected Ron's judgment, that could be a reason too.
One reason could be that Ron abandons Harry when he needs him the most. Like in a dangerous situation, if Ron runs away leaving Harry alone to face the threat, Harry would find it hard to forgive such cowardice.
Perhaps there were some secrets that Ron was keeping from Harry. In the wizarding world, secrets can be a big deal. If Ron had a secret related to his family or some other important matter and Harry found out about it in a way that made it seem like Ron was hiding something bad, it could lead to Harry not trusting him. Also, Ron's friendship with other characters might make Harry jealous or suspicious. If Ron was getting closer to someone Harry didn't trust, it could spill over and make Harry question Ron's loyalty to him.
One possible reason could be jealousy. Maybe Ron is jealous of Harry's fame and the attention he gets. Another reason might be a misunderstanding. For example, if Harry makes a decision without consulting Ron in a crucial situation, Ron could feel left out and get angry, leading to a fight.
One reason could be envy. Hermione is often very good at academics and magic, and Harry and Ron might be envious of her success. For instance, if Hermione always gets top grades in potions class while Harry and Ron struggle, they could lash out at her out of frustration.
One reason could be jealousy. Maybe Ron feels that Harry is getting too close to Hermione and he's afraid of losing her attention. In fanfiction, authors often play with the characters' emotions. Harry, on the other hand, might see Ron as being too possessive of Hermione.
One reason could be a divergence from the original friendship dynamic in the books. In the canon, Harry and Ron are good friends, and fanfiction that shows Harry hating Ron might go against what many fans consider the essence of their relationship.
One possible plot could be that they have a major disagreement over a Quidditch match. Maybe Ron blames Harry for losing the match because Harry made a risky move that didn't pay off. This causes a rift between them, and they start to see each other as rivals rather than friends. They compete in everything from academics to getting the attention of a certain teacher.