In the 'ash's betrayal pokemon fanfiction', the main antagonist could be an internal force within Ash himself. His own ambition and thirst for power could be personified as an antagonist. This 'inner self' might overtake his rational thinking and moral compass, causing him to betray those closest to him.
Perhaps an old enemy of Ash, like Team Rocket, but in a more devious way. They might have a new plan where they convince Ash that his friends are holding him back from achieving true greatness, leading him to betray them.
It could be a new character, someone who has a long - standing grudge against Ash's group. This character might manipulate Ash into betraying his friends by preying on his insecurities or desires.
It could be a powerful and evil Pokemon trainer who wants to capture Ash in his new Pokemon form. This trainer might be after Ash because he believes that a human - turned - Pokemon has some special power or value.
Maybe a powerful and evil pokemon trainer who has a vendetta against Ash. Now that Ash is in a vulnerable pokemon form, this trainer could try to capture and control him. The trainer might have some dark motives related to using Ash's unique situation to gain power in the pokemon world.
Team Rocket could still be the main antagonists. Even though they usually have comical failures, in this fanfiction, they could discover Ash's connection to the legendary Pokemon and try to use it for their own evil plans. Maybe they want to capture the legendary Pokemon through Ash or steal the power that Ash has because of his chosen - one status.
A powerful and greedy Team Rocket member could be the main antagonist. They might be after Diancie because of its rare and valuable powers. They could try to capture Diancie and use it for their own evil purposes, like selling its powers or using it to take over the Pokemon world.
A wealthy and power - hungry trainer who wants to stop Ash from completing his Nuzlocke journey. This trainer believes that the Nuzlocke rules are too restrictive and wants to prove that his way of training Pokemon is superior.
A new, powerful and evil Pokemon trainer could be the antagonist. This trainer might be jealous of Ash's new form and powers as a Pokemon and wants to capture him to use his powers for his own gain. He could set up various traps and battles to try and subdue Ash.
It could be a jealous rival trainer. This trainer might have always been envious of Ash's popularity and the loyalty of his harem. So, they plot and scheme to turn the harem against Ash by spreading lies and creating discord among them.
It could be a rival trainer who is jealous of Ash's success and also wants to use Princess Sara for her influence. He spreads false rumors about Ash to turn the people of the princess's kingdom against him. He then tries to take over the role of the hero in the situation, but his real intentions are to gain power and wealth by any means necessary.
It could be Team Rocket. They are always after rare and powerful pokemon. In this fanfiction, they might have a new and devious plan to steal the legendary pokemon that Ash is trying to catch. Their comical yet persistent attempts to thwart Ash would add an interesting dynamic to the story.
Maybe a new villain organization that believes in a different way of training Pokemon, a cruel and unethical way. They see Ash as a threat to their ideology because he is a symbol of the traditional, kind - hearted Pokemon training. So they constantly try to stop him from achieving his goal of becoming a Pokemon master. For example, they might try to steal his Pokemon or sabotage his battles.