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How to Design a Primer for a Novel Genetic Marker?

2024-10-11 19:27
3 answers

Designing a primer for a new genetic marker involves several steps. You have to analyze the sequence, consider factors like primer length and specificity. It's also important to make sure the primers anneal properly during the PCR process.

Well, to design a primer for a novel genetic marker, you first need to have a clear understanding of the target sequence. Then, you can use specialized software or online tools to help with primer design based on certain parameters like melting temperature and GC content.

The process of designing a primer for a novel genetic marker isn't straightforward. You need to take into account things like avoiding secondary structures and ensuring complementarity. There are many resources and guidelines available to assist you in this task.

Sir, How About A Marriage?

Sir, How About A Marriage?

At the peak of her career, A-list actress Song Ning announced her withdrawal from the entertainment industry for love, shocking the nation. Everyone thought that she must have found her ideal home. That was why she was so determined. At first, Song Ning thought so too. For the rest of her life, she would not be a celebrity. She would only be a virtuous and virtuous woman who would take care of her husband and children at home. However, on the night before their wedding, she found out that her fiancé was having an affair with her best friend. Enraged, Song Ning found a random man to register their marriage at the entrance of the Civil Affairs Bureau. She originally wanted to take revenge on her scumbag fiancé, but she did not expect that the man who registered his marriage with her was the heir of nation's largest financial group, Mu Chen. After they got married, Mu Chen doted on Song Ning and protected her in every way possible. He didn't allow anyone to bully her. Song Ning always thought that she would be happy for the rest of her life and live the best life she wanted. That's right, she got it. It was just a little different from what she had originally imagined. The person who gave her everything was someone else. Many years later… Song Ning looked at Mu Chen affectionately. "I'm really lucky. Thank God I met you and saved me from hell." Mu Chen smiled faintly. “Yes, thank God.” However, Song Ning would never know. Mu Chen wasn't talking about thanking God for letting him meet Song Ning. He was thanking God for letting Song Ning's fiancé cheated on her so that he would have a chance. There was no such thing as an accidental encounter. It was just a premeditated pursuit. That day, he waited for Song Ning outside the Civil Affairs Bureau for ten hours…
4.4
889 Chs
Her Wicked CEO: How To Seduce A Hikikomori Wife?

Her Wicked CEO: How To Seduce A Hikikomori Wife?

(Completed) Is there hate at first sight? Takeuchi Rie is a hikikomori who rarely goes sees the daylight. If she wants food, she will order it online. The love of her life is a manga character. Unless her house is on fire, she refuses to go outside. Kamiyama Kiyohira is the arrogant and proud CEO of Kamiyama Groups. When he meets her for the first time at the funeral of his father and stepmother, she is cursing him to rot with genital diseases at his father's funeral. He breaks her door, destroys her house, and nearly sends her to the prison. She sticks to him like a koala and blames him for taking advantage of her during sleep. They certainly hate each other, but they can't keep their hands off each other? He is the wicked CEO. She is the hateful Hikikomori. She is the wicked Hikikomori. He is the hateful CEO. Who is the wicked one here? It depends. Who is at the bottom and who is on the top? They are quite a match in words, bed, and hate. They hate each other so much that they keep sleeping with each other? By the way, if MC and ML are mutually sexually harassing each other, is it sexual harassment? ----------------- Author's Note: Not the usual CEO love story. Warning: Explicit language, 18+ scenes, NSFW *HIKIKOMORI = An acute antisocial person/ Shut-in/ A person who rarely goes outside. ----------------- The interview with The Wicked CEO: Author: How do you draw a Hikikomori out? CEO: Demolish her house. Author: How do you trick a Hikikomori to sign a legal document? CEO: Use difficult vocabulary. If she didn't study hard in school, she won't understand a word. Author: I see. How do you make her so mad that she tries to kill you right after she wakes up? CEO: Tell her that you have slept with her. Make sure that she misunderstands. Author: How do you seduce a Hikikomori? CEO: Read the novel and find out. Hikikomori: How dare you throw me in bed with my enemy? Come here, Author. I won't beat you to death. Definitely, won't beat you to death. Author: Have fun in bed. Mr CEO drags his Hikikomori wife away before she kills the author. ----------------------------- Proofreader/Editor: TheTalkingCupcake Picture credit: Photo by Joel Overbeck on Unsplash Instagram: Koch_Norah Goodreads: http://www.goodreads.com/norah_koch ****Full list of my novels******* Novels with Interconnected Worlds: 1. The Love That Remains (Completed) 2. His Mischievous Time-Traveler: How to capture a celebrity husband? (Completed) 3. His Devious Harbinger: How To Tame A Wicked God? (Completed) 4. Supernatural Investigation 101: Chronicles of Nakashima Wakana  5. When A Ghoul Falls In Love 6. Hunting for a Husband in a Vampire Apocalypse Standalone Novels:  Her Wicked CEO: How To Seduce A Hikikomori Wife? Miss Chicken & Her Mister The Villainous Emperor is My Pet? (Available on Goodnovel.Com) Bad x Bad: My Dear Hana (Available on Goodnovel.Com)
4.6
321 Chs
A Song For A Summer's Night

A Song For A Summer's Night

[R18: Completed] Two people, the CEO of an Entertainment company, Song Luli, and Long Jie, the successor of a conglomerate, are entangled together in a web of power, high society, and calculating schemes—and what do they need to do to survive it? A marriage pact to bind them together. Both are bound to duty, responsibility, and their families, and neither cares about love. But what happens when they both mutually respect and admire each other’s work? What happens when they cannot deny their physical attraction for long? Will their feelings develop profoundly, or will they maintain a platonic business relationship? Disclaimer: This novel is RESTRICTED to 18+ only due to Mature themes such as DETAILED SEX SCENES, COARSE LANGUAGE, AND FIGHT SCENES. THERE IS NO RAPE. ... Synopsis: Song Luli is a sharp-tongued, bold, and reserved young woman who unexpectedly becomes the new successor of her father's company—Song Entertainment—right after her older sister abruptly disinherits herself. Song Luli, now the heiress of a large company, soon receives word that Song Entertainment is crumbling in the business world. To secure her position, she must marry Long Jie, a wickedly handsome heir of a conglomerate, who is bound to duty and responsibility. Long Jie is a handsome, ruthless, and calculative young man who destroys careers or makes them, and he is known to be frigid by rejecting every offer for a suitable match. But he picks Song Luli. On the terms of marriage, they agreed to never fall in love, to never think about the possibility as they dedicate their time towards their jobs and families. Marriage benefited both of them for the sake of power and to survive the turmoils of High Society. Watch as the budding romance blooms between two stubborn and cynical individuals who share more in common than they want to admit, learning more about what it means to be married, and battle the power struggles that they face, together. Meanwhile... Song Luli's cousin Su Xiang is an aspiring doctor devoted to her job and inheriting the legacy her mother built. She is the heiress of Song Pharmaceuticals, one of the largest medicinal companies in the country. But when her older adoptive brother returns from four years of Medical school, she must confront the blooming feelings she harbours for him. ... Tags: #Arranged Marriage, #Romance, #Forbidden Romance, #Crime Drama, #Mystery, #Plot Twists, #Underworld *There are a few love stories interwoven in the plot that comes together in the end to unravel a devastating truth.* - No prolonged misunderstandings - No sexual assault Discord: https://discord.gg/nBT8jWj ... Art, not mine, will be removed at request. Credit to the artist!
4.8
147 Chs
I transmigrated to a mafia character from a BL Dark novel!

I transmigrated to a mafia character from a BL Dark novel!

Lee Fei is a normal 30-year-old man who has a good job as a librarian at the National University in China. He is an avid reader and has always read everything, including BL and GL, even though he declares himself 100% straight. He died just like that. There was no truck. There was no car accident. No deadly disease. Lee Fei reincarnated and transmigrated to the world of the book he had read days before he died. Unfortunately, a BL Dark Book. The worst, he transmigrated as an insignificant side character! Lee Fei became Emery Darcy, who is the third child of a powerful duke. Your life should be full of wealth and happiness, right? Unfortunately, Emery Darcy followed the path of an exorcist! Yes, Emery Darcy is an Exorcist Bishop! To make the irony of his situation worse, this side character had a worse fate than the villain. What an injustice! As the knowledge of the plot of this world, Lee Fei intends to return to the home of his rich family and live a good life! So, what if he has divine powers and MANA from nature? He just wants a rich and peaceful life! Ehhhh? Is the MC making a honey trap for me? Is ML no longer a possessive demon, but a tsundere that is following me? Is the fallen archangel a shameless voyeur who spies on me even in the bath? A guardian knight who thinks I'm the reincarnation of the god of nature? Stay away from me, I just want a peaceful life! Does apocalypse come far? No matter, I'm rich enough to protect myself! Let's have a happy and rich life as a good nobleman should be. NOTE: 1. R 18 2. Chapter Auxiliary with the main characters of the harem so far; 3. Harem Romance 4. Follow my Ko-fi for news and weekly calendars: https://ko-fi.com/post/Calendar-1001--1601-L4L27V5L9 and Ko-fi.com/take_the_moon. Thanks for your support in the 2021. Hope we're together in 2022 too.
4.5
226 Chs
What is the significance of the 5th novel marker?
2 answers
2024-10-06 00:57
The 5th novel marker might be a crucial plot point or a symbol that drives the story forward in a significant way.
What exactly is the dopamine novel marker?
2 answers
2024-10-02 02:59
The dopamine novel marker is a concept related to identifying specific elements or patterns in novels that potentially trigger dopamine release in readers.
How to write a primer for a comic book?
1 answer
2024-10-12 20:24
Start with a clear concept. Decide on the genre, theme, and main characters of your comic. Then, sketch out a rough storyline to guide your writing.
How to Remove Marker from Comic Book?
2 answers
2024-10-06 21:13
You can try using rubbing alcohol. Dab a little on a cotton swab and gently rub the marked area. But be careful not to damage the paper.
What generates novel genetic diversity? Is it a genetic drift?
1 answer
2024-09-29 07:36
Genetic drift can indeed generate novel genetic diversity. It happens when there's random fluctuation in the gene pool of a population. For instance, in a small isolated group, just by...
What are the key elements of a primer of the novel?
3 answers
2024-10-08 17:28
A primer of a novel usually includes an introduction to the main characters, a brief summary of the plot, and maybe some background information on the setting and themes.
Hai Bodong and Primer
1 answer
2024-10-19 01:22
Hai Bodong was a character in 'Battle Through the Heavens' and its derivative works, and he was the Primer clan's Supreme Elder. When he was young, Hai Bo Dong was...
Hai Bodong and Primer
1 answer
2024-10-17 18:48
Hai Bo Dong was a character in the fantasy novel 'Battle Through the Heavens' written by Tian Can Tudou, a writer from Qidian, and the works derived from it. He...
How to write a novel about genetic evolution?
1 answer
2024-09-01 10:45
Genetic evolution novels usually combined science fiction and biology to describe the changes and adaptation of humans or other organisms in the process of genetic evolution. Here are some writing...
How to remove permanent marker from comic books?
2 answers
2024-10-11 07:46
One way is to use white vinegar. Soak a cloth in it and then blot the marker stain. But be careful not to soak the comic book too much.
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