One way is to use specific search parameters and filters in BLAST to narrow down the results and look for proteins that haven't been previously identified or characterized.
Well, finding novel proteins in BLAST involves being precise with your input sequences and analyzing the output carefully. You might want to explore different BLAST algorithms and tweak the parameters based on your specific research needs. Sometimes, collaborating with experts or referring to relevant research papers can give you useful tips too.
To find novel proteins in BLAST, you can start by optimizing your query sequence and comparing it against a comprehensive database. Also, pay attention to the alignment scores and similarities to identify potential novel matches. Additionally, using advanced options and tools within BLAST can help increase the chances of discovering new proteins.